(Methods in Molecular Biology) 2015th Edition
by Alexandra Castilho (Editor)
Conceived with the intention of
providing an array of strategies and technologies currently in use for
glyco-engineering distinct living organisms, this book contains a wide
range of methods being developed to control the composition of
carbohydrates and the properties of proteins through manipulations on
the production host rather than in the protein itself. The first five
sections deal with host-specific glyco-engineering and contain chapters
that provide protocols for modifications of the glycosylation pathway in
bacteria, yeast, insect, plants and mammalian cells, while the last two
sections explore alternative approaches to host glyco-engineering and
selected protocols for the analysis of the N-glycans and glyco-profiling by mass spectrometry. Written for the highly successful Methods in Molecular Biology
series, chapters include introductions to their respective topics,
lists of the necessary materials and reagents, step-by-step, readily
reproducible laboratory protocols and tips on troubleshooting and
avoiding known pitfalls.
Authoritative and extensive, Glyco-Engineering: Methods and Protocols
offers vast options to help researchers to choose the expression system
and approach that best suits their intended protein research or
applications.
