(Methods in Molecular Biology) 2013th Edition
by Nobuyoshi Kosaka (Editor)
microRNAs (miRNAs) are small
non-coding RNAs that regulate various biological phenomena, such as
development and homeostasis. The dysregulation of miRNA leads to disease
progression, particularly of cancer. In Circulating MicroRNAs: Methods and Protocols,
expert researchers in the field detail recent advances in the
isolation, purification and analysis of circulating miRNAs from a
variety of sources for research. The book is divided into three main
topics. The first section involves the study of secretory miRNAs in
cell-cell communication, and the second, the study of circulating miRNAs
in body fluids. The last describes the novel techniques used to study
circulating miRNAs. Written in the highly successful Methods in Molecular Biology™
series format, chapters include introductions to their respective
topics, lists of the necessary materials and reagents, step-by-step,
readily reproducible laboratory protocols, and key tips on
troubleshooting and avoiding known pitfalls.
Authoritative and practical, Circulating MicroRNAs: Methods and Protocols seeks to aid scientists in dealing with the recent advances of RNAi technology from the bench to the bedside.
