(Springer Protocols Handbooks) 1st ed. 2020 Edition
by Alangar Ishwara Bhat (Author), Govind Pratap Rao (Author)
This book provides detailed information on methodologies used in
biological, serological and nucleic acid based assays for the detection,
diagnosis and management of plant viruses. The content is divided into
six main parts, the first of which presents techniques used in the
biological characterization and transmission of viruses, while Part II
covers purification and techniques concerning the physico-chemical
properties of viruses. In turn, Part III focuses on in vitro
expression, production of polyclonal and monoclonal antibodies; and on
various serological assays including precipitin tests, ELISA, blot
immunoassays, immunosorbent electron microscopy and lateral flow
immunoassays. Part IV addresses the isolation of DNA and RNA from plants
and nucleic acid based assays such as dot-blot, polymerase chain
reaction, real-time PCR, loop-mediated isothermal amplification, rolling
circle amplification, recombinase polymerase amplification, and
next-generation sequencing approaches. Part V discusses cloning,
sequencing, sequence analysis and the production of infectious clones,
while the last part (Part VI) provides biotechnological approaches for
the management of viruses. Given its scope, the book will be a valuable
resource for every laboratory, student and teacher, and for everyone
interested in plant virology, plant pathology, plant biology and
molecular biology, offering them a practical manual on various aspects
of plant viruses.
