BOOK DOWNLOAD

Barley: Methods and Protocols

10.00$
Barley: Methods and Protocols
BOOK DOWNLOAD

Barley: Methods and Protocols

10.00$

Author(s)Wendy A. Harwood
Year2019
Pages316
LanguageEnglish
FormatPDF
Size12 MB
PublisherHumana Press
ISBN1493989421, 978-1493989423

This detailed volume explores barley as both a crop and a model, with practical techniques such as crossing barley, a range of tissue culture methods, the preparation of barley tissues for different forms of microscopy, and the assessment of sensitivity to abiotic stresses. Efficient protocols are provided for transformation, TILLING, virus-induced gene silencing and genome editing. There is also particular emphasis on a range of protocols for genotyping and for the analysis of gene expression. Written for the highly successful Methods in Molecular Biology series, chapters include introductions on their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and easy-to-use, Barley: Methods and Protocols serves as a valuable reference volume for cereal researchers and breeders by providing detailed protocols covering important traditional skills such as crossing and tissue culture through to the latest technologies for genotyping, expression analysis, and genome editing.